This choice will determine, against which
reference genome the designs will be tested.
In the textarea may be entered either a
FASTA format sequences in nucleotide alphabet e.g:

>some sequence
ATATCGATGCTAGCTAGCTspacerGATCGTAGCTAGCTAGCTA

Or a FASTA format sequences in nucleotide alphabet e.g:

>some sequence
NN_NI_NI_NI_NI_NG_NN_NN_NI_NG_NI_NG_HD_NG_NG_NI_HDspacerHD_NK_NI_NG_NN_NG_HD_HD_HD_NI_NN_NN_HD_NN_HD_NN_NI

Caution: the word spacer has to be in between.
The file might contain
FASTA format sequences in nucleotide alphabet e.g:

>some sequence
ATATCGATGCTAGCTAGCTspacerGATCGTAGCTAGCTAGCTA

>some other sequence
ATATCGATGCTAGCTAGCTspacerGATCGTAGCTAGCTAGCTA

Or FASTA format sequences in RVD alphabet e.g:

>some sequence
NN_NI_NI_NI_NI_NG_NN_NN_NI_NG_NI_NG_HD_NG_NG_NI_HDspacerHD_NK_NI_NG_NN_NG_HD_HD_HD_NI_NN_NN_HD_NN_HD_NN_NI

Caution: the word spacer has to be in between.
It can be specified if the TALEN designs are be tested for any secondary off-target effects.
This means if they will cut in any exogenous, foreign introduced sequences.
Check every individual off-target or paste a set of FASTA sequences into the text area
This text area only accepts FASTA format sequences as input e.g.:
>some sequence
ATATCGATGCTAGCTAGCT
GATCGTAGCTAGCTAGCTA

1. Organism of interest:

[HELP]

select off-target database

2. Sequence input:
[HELP]






Alternatively upload a file in FASTA format

[HELP]



3. Secondary off-targets:

Test secondary off-target effects [HELP]

Click to choose which off-target sequences to test


4. Start job:


Show result as Genome browser image




Boutros lab, E-TALEN-Version 2.5
For suggestions please contact us at crispr@dkfz.de